Table I.
Peak positions and FWHMs for selected and activated hemoglobin peptidesa
| selection time (ms)b | peptidesc | tD2 (FWHM) |
|---|---|---|
| 8.236 | β-chain [L96-R104+3H]3+ | 13.24 (0.20) |
| 15.18 (0.20) | ||
| β-chain [V133-K144+3H]3+ | 14.17 (0.20) | |
| α-chain [V93-K99+2H]2+ | 14.36 (0.42) | |
| α-chain [V17-R31+3H]3+ | 15.04 (0.45) | |
| β-chain [L105-K120+4H]4+ | 15.18 (0.30) | |
| α-chain [T41-K56+4H]4+ | 15.25 (0.21) | |
| β-chain [S9-K17+2H]2+ | 15.37 (0.26) | |
| 9.366 | α-chain [V93-K99+2H]2+ | 13.96 (0.21) |
| 17.09 (0.26) | ||
| β-chain [E121-K132+3H]3+ | 15.24 (0.15) | |
| α-chain [V17-R31+3H]3+ | 15.04 (0.21) | |
| 16.43 (0.21) | ||
| 17.04 (0.31) | ||
| α-chain [M32-K40+2H]2+ | 17.13 (0.39) | |
| 17.59 (unresolved) | ||
| β-chain [V67-K82+3H]3+ | 17.44 (0.46) | |
| β-chain [L96-R104+2H]2+ | 17.13 (0.31) | |
| α-chain [T41-K56+3H]3+ | 17.11 (unresolved) | |
| 18.45 (0.26) | ||
| 10.908 | α-chain [T41-K56+3H]3+ | 16.99 (0.15) |
| 19.00 (0.23) | ||
| 20.69 (0.15) | ||
| β-chain [G83-K95+2H]2+ | 19.73 (0.26) | |
| β-chain [V18-R30+2H]2+ | 20.02 (0.36) | |
| α-chain [F128-K139+2H]2+ | 19.67 (0.13) | |
| 20.18 (0.31) | ||
a
All activations were carried out with IA2 = 65 V.
b
Selection time is when a 150 µs wide pulse was applied to G2 to transmit ions.
c
Peptide sequence listed as N-terminal residue to C-terminal residue, including information about their position in the sequence of the intact protein.
d
Note: if no shift occurs, tD2 ≈ 1.8 × tD1. Because the time the precursor spent in the ion funnel is subtracted to obtain tD2, the relation is not exact.