Figure 2. Targeting emmprin suppresses KSHV promotion of cell invasiveness.
Cells were transfected with either control non-target (n-siRNA) or emmprin-specific siRNA (e-siRNA), and after 48 h, HFF (A) or HUVEC (C) were incubated with media (mock) or KSHV (K) for 2 h. Subsequent transwell invasion assays were performed 24 h later, and invasiveness for HFF (B) and HUVEC (D) in each group was determined relative to control cells as in Methods. Error bars represent the S.E.M. for three independent experiments. ** = p<0.01 for comparison of invasion for e-siRNA-transfected/KSHV-infected cells versus n-siRNA-transfected/KSHV-infected control cells.