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Purification of the scFv 1E8a by Ni-NTA chromatography. a, Western blot probed with an anti-His6 Ab that revealed the presence of the His6-tagged scFv 1E8a. b, SDS-polyacrylamide gel electrophoresis stained with Coomassie blue, PE, flow-through (FT), wash fraction (W), and the elution of the purified scFv protein (see Materials and Methods).
