Fig. 4.

Emetine-mediated inhibition of HIF-dependent transcription. A, 786-O cells were untreated or exposed to the indicated concentrations of emetine for 4 h, and mRNA expression for GLUT-1 and VEGF were analyzed by quantitative RT-PCR. Results shown are representative of three separate experiments. B and C, 786-O cells were untreated or exposed to the indicated concentrations of emetine or CHX for 24 h in serum-free medium. The conditioned media were subjected to a VEGF ELISA (B), and TGF-α protein was measured in whole-cell lysates with a TGF-α ELISA (C). Statistical significance was calculated using SigmaPlot 9.0 (*, p < 0.05). Each bar represents the mean ± S.D. (n = 3).