Table 1. Mean Change in Assay Results by Handling Condition.

Days incubated prior to testing	1		3		7		15
	N	Mean Change (SD)	N	Mean Change (SD)	N	Mean Change (SD)	N	Mean Change (SD)
BED assay (OD-n)
Storage at 4°C	30	−0.05 (±0.59)	31	−0.01 (±0.23)	31	0.02 (±0.26)	27	−0.01 (±0.31)
Storage at 25°C	30	0.02 (±0.25)	32	−0.03 (±0.22)	31	−0.02 (±0.12)	27	−0.05 (±0.23)
Storage at 37°C	31	−0.02 (±0.21)	28	−0.19 (±0.29)†	19	−0.34 (±0.27)†	6	−0.54 (±0.20)‡
Vironostika-LS assay (SOD)
Storage at 4°C	31	0.08 (±0.33)	31	0.01 (±0.45)	31	0.05(±0.54)	8	0.11 (±0.58)
Storage at 25°C	30	0.13 (±0.41)	31	0.06 (±0.40)	30	0.07(±0.50)	8	−0.16 (±0.36)
Storage at 37°C	30	0.02(±0.45)	28	−0.21 (±0.50)‡	19	−0.71 (±1.33)‡	3	−0.96 (±0.33)‡
Avidity assay (%)
Storage at 4°C	31	1.71 (±5.67)	31	0.36 (±8.17)	30	−1.75 (±18.9)	8	−3.41 (±10.0)
Storage at 25°C	31	−0.29 (±7.20)	31	0.89 (±4.71)	30	−3.38 (±19.1)	8	−2.54 (±8.32)
Storage at 37°C	31	−0.34 (±4.98)	28	−1.41 (±4.90)	14	−7.71 (±18.0)	3	−1.99 (±1.04)+
SD: Standard Deviation. Paired t-test: †p<.05, ‡p<.01, +p  =  .08

SD: standard deviation; OD-n: normalized optical density; SOD standardized optical density;

^†P value<0.05.

^‡P value<0.01.

⁺P value = 0.08.