Table 1. Assigned threshhold scores using the Huesken dataset to analyse each scoring tool to two thresholds to filter siRNA with expected inhibition efficiency 90%.

	Min score	Min Threshold	Mean	Max Threshold	Max score	Standard Deviation
Reynold	0	1.9	5.52	9.15	10	1.81
Ui-Tie	III	III	Ib	Ia	Ia	0.84
Amarzguioui	−2	−1.21	2.04	5.3	5	1.62
Katoh	31	42.03	69.52	97.01	103.9	13.7
Hsieh	−2	−1.11	1	3.11	4	1.05
Takasaki	−11	−10.22	1.92	14.06	20.2	6.07

First, siRNA with inhibition efficiency above 90% are isolated from the dataset. Then for each scoring tool, the mean and Standard deviation is calculated and the minimum and maximum thresholds are assigned by deviation from the mean by two folds of standard deviation.