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. 2011 Oct 26;6(10):e26278. doi: 10.1371/journal.pone.0026278

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Bijtenhoorn et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A) Physiological map of the insert of the metagenome derived clone Bio5 (3.1 kb, GenBank # EF530730.1); open reading frames identified are indicated as arrows in the direction of transcription. A potential Shine-Dalgarno sequence at the 5′-end of bpiB09 is underlined; the potential translational start site of bpiB09 is in bold; B) SDS-PAGE of recombinant BpiB09 after his-tag purification. Lane 1: 2 µg BpiB09; Lane 2: molecular weight marker (kDa).