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. 2011 Oct 26;6(10):e26521. doi: 10.1371/journal.pone.0026521

Figure 6. Validation of three predicted EMT-regulating miRNAs.

Figure 6

(A) The EMT-related miRNA subcluster derived by using either positive (upper) or negative (lower) sPCC values as described in Figures 4 and S3. (B and C) Ability of miRNAs in the epithelial cluster to cause upregulation of E-cadherin in mesenchymal ACHN cells. ACHN cells were either transfected (B) once or (C) 3 times with the indicated miRNAs, and after (B) 3 days and (C) 12 days respectively, the expression of E-cadherin mRNA was quantified by real-time PCR. The Western blot inserted in C confirmed that the upregulation of E-cadherin mRNA also resulted in a induction of E-cadherin protein. (D) Comparison of the expression of the three new EMT-regulating miRNAs between the epithelial (E) and mesenchymal (M) cell lines among the NCI60 cells recently described [11].