Table 1. Thermodynamic parameters for binding of DST with chromatin, chromatosome.

	Temperature (°C)	N (drugs/base)	ΔH (Kcal/mol)	ΔS (e.u)	Ka(×105)M−1	ΔG (Kcal/mol)	ΔCp (calK−1 mol−1)
Chromatin	10	0.15±0.002	−7.35±0.13	1.49	9.99±1.03	−7.77	−120.8±11.4
	15	0.15±0.002	−7.72±0.11	−0.465	5.63±0.38	−7.58
	20	0.13±0.003	−8.51±0.31	−2.76	5.50±0.84	−7.70
	25	0.14±0.002	−9.10±0.16	−4.48	4.89±0.37	−7.76
Chromatosome	13	0.11±0.002	−5.26±0.15	7.79	5.25±0.57	−7.49	−208.7±42.9
	17	0.09±0.006	−6.78±0.60	1.51	2.74±0.52	−7.22
	21	0.11±0.005	−6.98±0.38	1.21	2.80±0.32	−7.33
	25	0.09±0.006	−7.98±0.62	−2.96	1.61±0.17	−7.10
Chromosomal DNA	10	0.10±0.002	−11.49±0.38	−13.5	8.42±0.96	−7.67	−45.4±18.3
	15	0.12±0.004	−11.39±0.58	−13.6	4.53±0.83	−7.47
	20	0.13±0.003	−11.68±0.29	−13.6	5.46±0.63	−7.69
	25	0.14±0.002	−12.15±0.22	−14.8	4.75±0.38	−7.74

^aExperiments were performed in 5 mM Tris HCl (pH 7.4), 15 mM NaCl at the temperatures stated in the table.