Figure 4. CD4 T cell help enhances, but is not required, for the maintenance of gp100-specific CD8 memory T cells.

(A) Schematic diagram of treatment schedule. (B & C) Mice received 10⁴ naïve CD8⁺Thy1.1⁺ (pmel) cells one day prior to treatment as in Panel A, and the proportion of Thy1.1⁺ (pmel) cells among live CD8⁺ cells was determined 60 days (B) or 90 days (C), following surgery. All analyses were performed on vitiligo-affected mice; although unaffected mice were used as a negative control on day 60. Dot plots depicting representative mice are shown. Symbols represent individual mice, and horizontal bars represent averages. Statistically significant differences were assessed by t test as indicated by brackets, NS denotes P>0.05. Data represent 3 combined experiments.