Table 1. Fungal phylotypes recovered in this study, detailing best BLAST matches to unpublished 454 data from marine sediments [19] and top-scoring hit amongst published sequences in GenBank.
| Marine 454 Data* | Top Scoring BLAST Mach in GenBank | |||||||||||
| Fungal Sequence | Primers | Length | Nematode taxa | SequenceIdentity | Gaps | E-value | Query coverage | Sequence Identity | Gaps | E-value | Query coverage | Taxonomic Identity |
| DS1 | F04/R22 | 356 bp | Southerniella sp. | 100% | 0/385 | 0.0 | 99% | 100% | 0/390 | 0.0 | 100% | Uncultured fungus clone(Acc GU072590) |
| DS2 | F04/R22 | 404 bp | Halalaimus spDesmodora sp. | 98% | 1/399 | 0.0 | 99% | 100% | 0/404 | 0.0 | 100% | Uncultured Agaricomycotina clone(Acc EU647009) |
| DS3 | F04/R22 | 399 bp | Metadasynemella sp.Chromaspirinia sp.Amphimonhystrella sp. | 98% | 3/395 | 0.0 | 99% | 99% | 0/399 | 0.0 | 99% | Seiridium sp(Acc AF346558) |
| DS4 | F04/R22 | 380 bp | Campylaimus sp. | 95% | 5/399 | 0.0 | 99% | 99% | 0/402 | 0.0 | 100% | Cryptococcus sp.(Acc DQ645520) |
| DS5 | F04/R22 | 397 bp | Desmoscolex sp. | 99% | 0/392 | 0.0 | 99% | 99% | 0/397 | 0.0 | 99% | Fungal sp.(Acc GQ120167) |
| DS6 | F04/R22 | 399 bp | Daptonema sp. | 99% | 1/395 | 0.0 | 99% | 100% | 0/399 | 0.0 | 100% | Uncultured ascomycete clone(Acc EU409874) |
| DS7 | F04/R22 | 390 bp | Molgolaimus sp. | 100% | 0/385 | 0.0 | 99% | 100% | 0/390 | 0.0 | 100% | Uncultured fungus isolate(Acc FJ785876) |
| DS8 | F04/R22 | 404 bp | Desmodora sp. | 98% | 1/399 | 0.0 | 99% | 100% | 0/404 | 0.0 | 100% | Uncultured Agaricomycotina clone (Acc EU647009) |
| RH1 | F04/R22 | 397 bp | Daptonema sp.Daptonema normandicum | 100% | 0/392 | 0.0 | 99% | 100% | 0/397 | 0.0 | 100% | Uncultured fungus(Acc AJ965493) |
| RH2 | F04/R22 | 398 bp | Terschellingia sp.Terschellingia longicaudata Parodontophora sp.Viscosia viscosa Sabatieria pulchra | 97% | 2/394 | 0.0 | 99% | 99% | 0/398 | 0.0 | 100% | Uncultured marine fungus clone(Acc GQ120110) |
| RH3 | F04/R22 | 390 bp | Viscosia viscosa Sabatieria pulchra | 99% | 0/385 | 0.0 | 99% | 99% | 0/390 | 0.0 | 100% | Uncultured eukaryote(Acc AB510380) |
| D9 | F22/R24 | 425 bp | Ceramonematidae sp. | N/A | N/A | N/A | N/A | 100% | 0/425 | 0.0 | 100% | Monascostroma innumerosum(Acc GU296179) |
| D10 | F22/R26 | 523 bp | Acantholaimus sp. | N/A | N/A | N/A | N/A | 99% | 0/522 | 0.0 | 99% | Vuilleminia comedens strain T-583 (Acc AF518594) |
| D11 | F24/R13 | 542 bp | Diplopeltula sp. | N/A | N/A | N/A | N/A | 99% | 0/542 | 0.0 | 99% | Uncultured fungus clone(Acc AB469139) |
| D12 | F24/R13 | 478 bp | Desmoscolex sp. | N/A | N/A | N/A | N/A | 100% | 0/478 | 0.0 | 100% | Uncultured eukaryote clone(Acc EU682622) |
Some 18S regions were not represented in 454 datasets (denoted by N/A).