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. 2011 Oct 26;6(10):e26636. doi: 10.1371/journal.pone.0026636

Figure 3. Lipid-deficient Wnt1 does not accumulate on the surface of L cells.

Figure 3

L cells stably transfected with wild-type and mutant Wnt1 constructs were treated with 100 ug/ml of heparin to release Wnt1 protein bound to HSPGs. After labeling cells with Sulfo-NHS-LC-biotin, cell surface proteins (S) were immunoprecipitated using NeutrAvidin beads. Aliquots of the initial lysates (L; before immunoprecipation) and the immunoprecipitated material were subjected to SDS-PAGE, blotted onto PVDF, and probed with the indicated antibodies. We used Flotillin (cell surface) and ß-tubulin (intracellular) as internal controls. Blots were scanned and quantitated using NIH ImageJ. This experiment was replicated 6 times. Error bars represent the standard error.