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. 2011 Oct 26;6(10):e26776. doi: 10.1371/journal.pone.0026776

Figure 6. Regulation of netrin-1 production by glucose and protein (albumin) in TKPTS cells.

Figure 6

A. Increasing concentration of D-glucose or mannitol was added to confluent culture. Twenty-four hours after addition the supernatant was harvested. Netrin-1 was quantified by ELISA. D-glucose down-regulated netrin-1 production in a dose-dependent manner whereas D mannitol did not alter netrin-1 production. *, p<0.001 vs. mannitol group. n = 4. B. Bovine serum albumin (BSA) (20 mg/ml) was added to confluent culture with/without U0126. Twenty-four hours after addition supernatant was harvested. Netrin-1 was quantified by ELISA. BSA significantly increased netrin-1 production, which was suppressed in the presence of U0126. *, p<0.001 vs. control. #, p<0.001 vs. BSA. n = 6.