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. 2011 Oct 26;6(10):e26831. doi: 10.1371/journal.pone.0026831

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Tan et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Alignment of BnCLIP1. The predicted plastid transit peptide is underlined, and the GxSxG lipase motif is marked with a box. The three strictly conserved amino acids Ser³⁰⁴, Asp³⁶⁷, and His⁴²³ are marked with dark arrows forming the putative catalytic triad. The BnCLIP1 sequence was aligned against three chloroplast-localized lipases AAD24845, DAD1, and DGL (GenBank Acc. Nos. AAD24845, NP_182008, and ACA48222), respectively. (B) Phylogenetic analysis. Phylogenetic tree was constructed using the sequences of BnCLIP1, AAD24845, DAD1, and DGL, and microorganism lipases 1TGL_A and RNL (GenBank Acc. Nos. 1TGL_A and P61871, respectively). BnCLIP1 is indicated by a black block.