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. Author manuscript; available in PMC: 2012 Nov 24.
Published in final edited form as: Neuroscience. 2011 Sep 8;196:35–48. doi: 10.1016/j.neuroscience.2011.08.044

Fig 1.

Fig 1

A. RNA expression profiles on the non-transfected and rat-NK3-transfected CHO cell lines. NK3-R RNA was only present in the rNK3-transfected cells. The CHO cells do not express the NK1R, the NK2R, or neurokinin B (NKB), the other members of the tachykinin receptor family. B. Changes in intracellular [Ca++] in non-transfected CHO cells or CHO cells transfected with the rat (rNK3) or human (hNK3) NK3-Rs in response to 1nM senktide. [Ca++]i was monitored with Fura-2 AM and the ratio of fluorescence emitted in response to excitation at 340 and 380 nM is presented. Senktide reached the cells at slightly different times in the 3 preparations (Frame 26 for non-transfected cells; Frame 32 for hNK3 cells; Frame 34 for rNK3 cells). Data represent the mean ratio of 340nm/380nm responses in 24–29 cells analyzed/culture.