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. Author manuscript; available in PMC: 2011 Oct 27.
Published in final edited form as: Cancer Res. 2010 May 1;70(9):3687–3696. doi: 10.1158/0008-5472.CAN-09-3931

Figure 1.

Figure 1

Assembly of S1MP-siRNA-DOPC. A, concept of multistage delivery system. B to D, Scanning electron microscopic images of S1MP at different magnifications. E, loading of Alexa555-siRNA-DOPC to the S1MP. After the loading, fluorescence from unincorporated Alexa555-siRNA-DOPC was measured to assess the loading efficacy. S1MP loaded with Alexa555-siRNA-DOPC were dissolved in 0.25% tetramethylammonium hydroxide and the loaded siRNA were separated by gel electrophoresis and visualized with SYBR Gold. F, release kinetics of Alexa555-siRNA-DOPC from the S1MP. The Alexa555-siRNA-DOPC–loaded S1MP were incubated in 10% FBS and the supernatant was separated to measure fluorescent intensity at Ex544/Em590 at different time points.