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. Author manuscript; available in PMC: 2011 Oct 27.
Published in final edited form as: Cancer Res. 2010 May 1;70(9):3687–3696. doi: 10.1158/0008-5472.CAN-09-3931

Figure 2.

Figure 2

Systemic delivery of siRNA-DOPC using S1MP results in long-lasting in vivo gene silencing. The mice (three mice per time point) bearing SKOV3ip1 orthotopic ovarian tumors were injected with S1MP-EphA2-siRNA-DOPC or left nontreated. A, the tumors were harvested at the indicated time points for Western blot to measure EphA2 expression levels. Thirty micrograms of tumor lysate were separated on a 10% SDS-PAGE and transferred on to a polyvinylidene difluoride membrane. The membrane was incubated with anti-EphA2 antibody overnight at 4°C. The membrane was tested for β-actin to confirm equal loading. B, densitometric analysis was performed to normalize EphA2 expression by β-actin. Data were expressed as % of normalized value to the nontreated. C, immunohistochemical analysis of EphA2 expression in the SKOV1ip3 tumor. Images were taken at original magnification of ×400.