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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Biomaterials. 2011 Jun 8;32(26):6183–6193. doi: 10.1016/j.biomaterials.2011.04.036

Fig. 3.

Fig. 3

Pre - osteoblast differentiation assay. Activity of rhBMP-2 released from the polyelectrolyte multilayer films is preserved. MC3T3-E1 cells were exposed to rhBMP-2 released from multilayer films, with the dose normalized per milligram of scaffold weight. Alkaline Phosphatase Assay demonstrates dose dependent early activation of bone differentiation cascade at Day 5. After 21 days of culture, Alizarin Red Stain confirms the presence of calcium deposits laid down during the differentiation process. Visual inspection of cultures after Alizarin red staining confirms a dose dependent presence of calcium deposits. (A, B and C) Alkaline phosphatase assay at day 3 on cells differentiated with different release formulations as depicted. (D, E and F) Alizarin red assay at day 14 on cells differentiated with different release formulation as depicted. Representative images of the alizarin red stain are below the bar graph in (D, E, F). A single factor ANOVA test allowed rejection of the null hypothesis for both assays; and a Tukey test between different groups was performed (s.d., n=9, ** p < 0.01; * p < 0.05; ns = not significant; all others p < 0.001).