Figure 3.
TAT-CD137Lct induced phosphorylation of Erk, p38, and Jnk and activaton of C/EBP and CREB. (A) Peritoneal macrophages (1×106) were treated with 50 ng of indicated proteins and harvested at 0, 15, 30, 60, and 120 min after their treatment. Lysates (10 µg) were used for immunoblot. LPS (50 ng/ml) is used as a positive control. p-, phosphorylated. (B, C) Peritoneal macrophages (1×105) were treated with TAT-CD137Lct (20 ng) in the presence of PD98059, SB203580, or SP600125 (10 µM each) and cultured for 4 h. Levels of IL-6 (B) and TNF (C) in culture medium were measured using a CBA kit. Data are the mean±SD of triplicates. (D) EMSA for nuclear extracts from peritoneal macrophages stimulated with 50 ng of indicated proteins was performed with radiolabeled C/EBP and CREB probes. LPS (50 ng/ml) is used as a positive control.