Figure 1.

(A) Effect of different concentrations of IGF-I on TLR4 protein expression in differentiating C2C12 skeletal muscle cells. Cells were treated with IGF-I for 24 hr. (B) TLR4 protein expression in C2C12 cells treated with IGF-I (200 ng/ml) for 24 hr in the absence or presence of LY294002 (100µM) and Wortmannin (50 nM). (C) Cells were treated with IGF-I (200 ng/ml)+PD98059 (0.1 or 1.0 µM) for 24 hr. (D) Cells were treated with IGF-I (200 ng/ml)+SB203580 (0.1 or 1.0 µM) for 24 hr.