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. 2011 Aug 31;11(4):223–226. doi: 10.4110/in.2011.11.4.223

Figure 2.

Figure 2

(A) TLR4 mRNA expression determined by real-time PCR in C2C12 myotubes cultured for 24 hr with different concentration of IGF-I. Effect of LY294002 (B), Wortmannin (C), SB203580 (D), or PD98059 (E) on IGF-I-mediated TLR4 expression. (F) The mRNA expression level of IL-6 and TNF-α in C2C12 cells cultured for 24 hr in the absence or presence of IGF-I (200 ng/ml). Target mRNA values are normalized to the GAPDH mRNA level for each sample. Samples were analyzed in duplicate in parallel with GAPDH. Values are menas±SE of three independent experiments. *p<0.05 vs. Control.