Figure 1.

Loss of Hif-α Does Not Initiate Renal Cyst Formation

(A) Representative blots of the PCR amplification of genomic DNA from tails and kidneys of mice lacking combinations of Fh1, Hif-1α, Hif-2α, Phd1, Phd2, and Phd3 alleles. These show that Phd1 and Phd3 are constitutively deleted, whereas null alleles for Fh1, Hif-1α, Hif-2α, and Phd2 are present only in DNA from the kidney, generated as a consequence of excision of floxed alleles by Ksp-cre in the tubules.

(B) H&E staining of kidney sections from control, Hif-1α^−/−, Hif-2α^−/−, and Hif-1α^−/− Hif-2α^−/− mice showing that there is no renal cyst development by 40 weeks of age; scale bar = 100 μm.