Table 1.

Cysteine Residues in KEAP1 Found to Be Succinated in FH^−/− MEFs by Tandem Mass Spectrometry

2SC Residue	Peptide Amino Acid Position	Peptide Sequence	Mascot Score	Enzyme
38	34–52	ASTECKAEVTPSQHGNRTF	49	Chymotrypsin
	32–45	MYASTECKAEVTPS	56	Elastase
	35–45	STECKAEVTPS	33	Elastase
151	144–152	SISMGEKCV	42	Elastase
	144–155	SISMGEKCVLHV	22	Elastase
	145–152	ISMGEKCV	13	Elastase
	146–152	SMGEKCV	24	Elastase
	146–155	SMGEKCVLHV	25	Elastase
241	233–245	SRDDLNVRCESEV	38	Elastase
288	282–293	QMQLQKCEILQS	39	Elastase
319	304–320	IFEELTLHKPTQVMPCR	47	Trypsin
	304–323	IFEELTLHKPTQVMPCRAPK	34	Trypsin
	308–319	LTLHKPTQVMPC	23	Elastase
	315–323	QVMPCRAPK	25	Elastase
	318–323	PCRAPK	15	Elastase
613	602–615	SGVGVAVTMEPCRK	51	Trypsin
	607–615	AVTMEPCRK	17	Elastase

Sequences for all detected peptide species covering the relevant modified cysteine are shown. The enzyme used to generate the peptide species and the MASCOT score obtained for each peptide is listed accordingly.