Figure 1.
A Yeast Model for Studying Pyruvate Kinase
(A) PYK expression levels. Quantification of a Pyk1p (upper panel)- or Pyk2p (lower panel)-specific peptide in Δpyk1Δpyk2 yeast expressing TEFpr-PYK1, TEFpr-PYK2, CYCpr-PYK1, or CYCpr-PYK2. Normalized peak intensities of >3 MRM transitions are presented as the relative expression level (%) compared to the concentrations in the wild-type strain BY4741. For both isoforms, levels from the CYC1 promoter (CYCpr) were around 20% of the expression levels from the TEF1 (TEFpr) promoter. Error bars, ±SD from normalization to three reference peptides.
(B) Pyruvate kinase activity of BY4741 (WT) and TEFpr-PYK1, TEFpr-PYK2, CYCpr-PYK1, or CYCpr-PYK2 yeast. Error bars, ±SD.
(C) Overview on the yeast models with varying PYK activity.