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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Gastroenterology. 2011 Aug 10;141(5):1696–1708.e2. doi: 10.1053/j.gastro.2011.07.045

Figure 4.

Figure 4

Ectopic expression of cagA in ImSt cells and effect on apoptosis and DNA damage. (A) Representative Western blot for CagA and β-actin in ImSt cells transfected with empty vector and CagA expression vector. (B–F) Cells were co-transfected with EGFP and cagA vectors, and data were analyzed from EGFP+ cells 24 h later. (B) SMO expression levels analyzed by flow cytometry. (C) Summary apoptosis data in cells ± the SMO inhibitor MDL 72527 (250 µM). (D) Representative dot plots of annexin V versus PI for apoptosis measurement. (E) Summary data for oxidative DNA damage. (F) Representative histogram for 8-oxoguanosine levels. In B, C, and E, n = 2 in duplicate; **P < .01, ***P < .001 versus cells transfected with empty vector; in C and E, §§P < .01 versus cells transfected with cagA vector.