Fig 5.

Effect of cav-1 knockdown on proteins distribution and transcripts level. (A) Sucrose gradient fractionation was performed to isolate low buoyant fractions enriched in caveolin proteins. Immunoblotting of fractions harvested from the top (low sucrose density) to the bottom (high sucrose density) show that H1R was excluded from caveolin-enriched fractions. (B) In resting cells, TACE appeared to localize both with low and high sucrose density membranes. By contrast, cav-1 down-regulation by RNAi induced displacement of TACE mainly from the caveolin-enriched membranes (lower panels). (C) Cav-1 immunoprecipitates were immunoblotted for TACE to evaluate protein–protein interaction. IgG served as negative control.