Skip to main content
. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Biochemistry. 2011 Oct 11;50(43):9255–9263. doi: 10.1021/bi201003a

Figure 4. Electron transfer from Fe protein to MoFe proteins with the nucleotide analog αγ-methylene ATP.

Figure 4

Shown is the absorbance at 430 nm plotted against the time after mixing Fe protein (75 μM) and MoFe proteins (20 μM) in one syringe with the nucleotide analog βγ-methylene-ATP (18 mM). Shown are β-188Cys MoFe protein and Fe protein mixed against βγ-methylene-ATP (β-188Cys + βγ-methylene-ATP); and wild-type MoFe protein and Fe protein mixed against βγ-methylene-ATP (WT + βγ-methylene-ATP).