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. 1984 Nov 12;12(21):8043–8058. doi: 10.1093/nar/12.21.8043

S1-hypersensitive sites in eukaryotic promoter regions.

T Evans, E Schon, G Gora-Maslak, J Patterson, A Efstratiadis
PMCID: PMC320272  PMID: 6095186

Abstract

We have examined by fine mapping the S1 nuclease-hypersensitivity of the 5' flanking regions of the human beta-globin and rat preproinsulin II genes and of the SV40 origin/enhancer region. In all cases S1-hypersensitive sites are located in known or presumed promoter/regulatory regions. Though a consensus DNA sequence is not evident, all of these sites reside in predominantly homopurine-homopyrimidine stretches. The alternate (non-B) DNA structure which is revealed by the enzymatic probe is a sequence-dependent feature of a short stretch of DNA, which is retained upon transplantation into a foreign environment. The alternate structure exhibits S1-nicking patterns uniquely different from those associated with the presence of Z-DNA.

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Selected References

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