Figure 4.

β_IV tubulin in vestibular epithelia. A. Transverse section of a utricle showing bright label in the supranuclear regions of hair cells (long arrows) and in supporting cells (short arrows). B Confocal view of a utricle whole mount in a focal plane below the apex showing label in hair cells and supporting cells. The arrow indicates label in a hair cell surrounded by supporting cell label. Confocal image, optical section thickness-0.53 µm. C Apical confocal view of a utricle whole mount showing label in kinocilia (one of which is indicated by the arrow), in hair cells, and in supporting cells. Optical section thickness-0.53 µm. D Isolated vestibular hair cell showing label for actin (phalloidin–red) in stereocilia and the cuticular plate, and label for β_IV tubulin (green) in the soma and in the kinocilium. The scale bars represent 8 µm for A and D, 25 µm for B and C.