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. 2003 Nov 12;4(4):565–578. doi: 10.1007/s10162-003-4002-5

Figure 4.

Figure 4

GTTR and immunolabeled gentamicin are both localized in the hair bundle. In immature hair cells (A,B), phalloidin-Alexa-660-labeled hair bundles (►) appear above the sensory epithelium. GTTR (A’) and immunolabeled gentamicin (B’) fluorescence also occurs above the sensory epithelium in the region of the hair bundle (►). Colocalization analysis of single optical planes of explants double-labeled with phalloidin-Alexa-660 (blue) and GTTR (A’’) or immunolabeled gentamicin (B’’) reveal white pixels, indicating that immature hair bundles (►) are colabeled with GTTR or gentamicin antibodies. The kinocilium of several hair cells labeled with GTTR can also be seen (→) in A’ and A’’ . In the central saccule, mature hair cells labeled with phalloidin-Alexa-660-labeled hair bundles (►) appear above the sensory epithelium (C,D). GTTR (C’) or immunolabeled gentamicin (D’) fluorescence also occurs above the sensory epithelium in the region of the hair bundle (►), and verified by the white pixels in the colorized images using colocalization analysis (C’’,D’’). The kinocilium of several mature hair bundles labeled with GTTR can also be seen (→) in C’ and C’’. All images are from explants incubated with 300 µg/ml GT/GTTR or unconjugated GT (and subsequently immunolabeled) for 30 min. Scale bars in µm.