Table 1.
Theoretical and observed xy and z-axis resolution for Bio-Rad MRC 1024 confocal system with a Nikon TE300 microscopes
| 512 × 512 | 1024 × 1024 | ||||
|---|---|---|---|---|---|
| Oil immersionobjective | Theoreticalxyresolutiona (nm) | Pixelsize (nm) | Observedresolution (nm) | Pixel size(nm) | Observedresolution (nm) |
| 40× (N.A. 1) | 257.5 | 474 | 490 | 237 | 350 |
| 40× + zoom | 257.5 | 200 | 280 | 100 | 260 |
| 60× (N.A. 1.4) | 184 | 316 | 330 | 158 | 230 |
| 60× + zoom | 184 | 100 | 230 | 100 | 230 |
| Theoretical xz resolutionb(nm) | Pixel size (nm) | Observed resolution (nm) | |||
| 40× + zoom | 390.1 | 100 | 600 | ||
| 60× + zoom | 199 | 100 | 230 | ||
Theoretical resolution formulae (Nikon): afor the xy resolution: ((λ/(2 × N.A.)) bfor the z-axis resolution ((λ × η)/2 × (N.A.)2) + (η/7 × N.A. × mag), where λ is wavelength (515 nm), η is immersion oil refractive index (1.515); N.A. is numerical aperture, and mag. is magnification.