Mean ASR thresholds (± SEM) as a function of age and stimulus frequency in C57BL (A) and CBA mice (B). The threshold was the lowest level at which the ASR differed (p < 0.05, one-tail) from the activity baseline. In the C57BL mice, the 16 kHz ASR threshold increased with age, except for a slight drop or plateau from 14 to 24 weeks of age. The only other significant effects of age were for 8 kHz, for the loss of sensitivity at 14 weeks followed by a recovery at 18 and 20 weeks of age. There were no threshold changes in the CBA mice. Compared with the CBA mice, the C57BL mice had lower thresholds for the 4 kHz stimuli but higher thresholds for the 16 kHz stimuli.