Figure 3.

Experimental results with tI1-FFL motifs. Microscopy images are used for illustration, accompanied by the quantitative data obtained from flow cytometry analysis of at least 200 000 cells from each transfected well. (A) Experimentally measured input–output response of tI1-FFL. Red curve corresponds to wild-type LacO; orange, dark cyan, green, and light green curves represent four mutants with gradually decreasing LacI binding; brown curve is the negative control. (B) Coefficient of variation for each of the constructs illustrated in panel (A) using identical color coding. (C) Representative microscopy images of the circuits and controls. (D) Processed flow cytometry data measured for six tI1-FFL constructs, showing mean output values and their s.d. in each bin. Source data is available for this figure at www.nature.com/msb.

Source data for Figure 3 ^{(111.5KB, xls)}