Table 1. Experimentally determined fluxes and 95% confidence intervals estimated from MFA model using [U-13C5]glutamine as a tracer.
| Reaction | Normal |
Transformed |
T/N | ||
|---|---|---|---|---|---|
| Flux | Confidence interval | Flux | Confidence interval | ||
| Detailed description, assumptions, complete results, and data are available in Supplementary information. Net flux: (υF−υR) ( → ) and Exchange flux: min (υF,υR) (↔). Some scaling and undetermined exchange fluxes (i.e., confidence intervals of infinity, Inf) are omitted for brevity. Comparison of fluxes (Transformed versus Normal) is described as U=up, D=down, NC=no change, and ND=no detectable. Fluxes are listed × 1014 mol/(cell × h). | |||||
| Glycolysis | |||||
| Glucext → G6P | 65.5 | 61.2, 69.9 | 83.1 | 77.6, 88.6 | U |
| G6P → F6P | 62.5 | 58.2, 66.9 | 82.1 | 77.6, 87.6 | U |
| G6P↔F6P | 4.1 | 0.0, Inf | 18.6 | 0.0, Inf | ND |
| F6P → DHAP+GAP | 64.2 | 59.9, 68.5 | 82.4 | 76.9, 88.0 | U |
| DHAP → GAP | 64.2 | 59.9, 68.5 | 82.4 | 76.9, 88.0 | U |
| DHAP↔GAP | 36.7 | 0.0, Inf | 13.1 | 0.0, Inf | ND |
| GAP → 3PG | 129.2 | 120.6, 137.9 | 165 | 154.0, 176.1 | U |
| GAP↔3PG | 0 | 0.0, Inf | 0 | 0.0, Inf | ND |
| 3PG → Pyr | 129.2 | 120.6, 137.9 | 165 | 154.0, 176.1 | U |
| Pyr → Lac | 114.9 | 105.9, 123.4 | 158.1 | 146.9, 169.2 | U |
| Pyr↔Lac | 300.5 | 0.0, Inf | 9.2 | 0.0, Inf | ND |
| Lac → Lacext | 114.9 | 105.9, 123.4 | 158.1 | 146.9, 169.2 | U |
| Pentose phosphate pathway | |||||
| 6PG → P5P+CO2 | 3.00 | 2.71, 3.29 | 1.00 | 0.90, 1.10 | D |
| P5P+P5P → S7P+GAP | 0.83 | 0.73, 0.93 | 0.17 | 0.13, 0.20 | D |
| S7P+GAP → F6P+E4P | 0.83 | 0.73, 0.93 | 0.17 | 0.13, 0.20 | D |
| P5P+E4P → F6P+GAP | 0.83 | 0.73, 0.93 | 0.17 | 0.13, 0.20 | D |
| TCA cycle | |||||
| Pyrmit+CO2 → OAA | 0 | 0.0, 3.2 | 0 | 0.0, 1.9 | NC |
| Mal → Pyrmit+CO2 | 1.8 | 0.9, 5.9 | 1.8 | 0.7, 4.7 | NC |
| Mal↔Pyrmit+CO2 | 2.8 | 0.0, 3.2 | 1.6 | 0.0, 1.9 | NC |
| Pyrmit → AcCoAmit+CO2 | 16.1 | 13.5, 19.1 | 8.6 | 7.6, 9.6 | D |
| AcCoAmit+OAA → Cit | 16.1 | 13.5, 19.1 | 8.6 | 7.6, 9.6 | D |
| Cit → AKG+CO2 | 10.1 | 9.1, 11.2 | 5.4 | 4.9, 6.0 | D |
| Cit↔AKG+CO2 | 3.3 | 2.7, 3.9 | 3.7 | 3.2, 4.2 | NC |
| AKG → Suc+CO2 | 12.1 | 10.6, 13.7 | 7.4 | 6.1, 8.7 | D |
| Suc → Fum | 12.1 | 10.6, 13.7 | 7.4 | 6.1, 8.7 | D |
| Suc↔Fum | 37.2 | 0.0, Inf | 0 | 0.0, 1.5 | ND |
| Fum → Mal | 12.1 | 10.6, 13.7 | 7.4 | 6.1, 8.7 | D |
| Fum↔Mal | 0 | 0.0, Inf | 42 250 | 1.5, Inf | ND |
| Mal → OAA | 10.3 | 6.7, 11.3 | 5.6 | 3.5, 6.1 | D |
| Mal↔OAA | 36.2 | 0.0, Inf | 57 630 | 3.2, Inf | ND |
| Pyr → Pyrmit | 14 | 12.0, 17.0 | 7 | 6.0, 8.0 | D |
| Pyr↔Pyrmit | 74 | 39.0, 133 | 276 | 87.0, 4566 | U |
| Glutamine metabolism | |||||
| Glnext → Gln | 20 | 19.0, 22.0 | 16 | 15.0, 17.0 | D |
| Gln → Glu | 20 | 19.0, 21.0 | 16 | 15.0, 17.0 | D |
| Glu → Gluext | 18 | 16.0, 19.0 | 14 | 12.0, 15.0 | D |
| Glu → AKG | 2 | 1.0, 3.0 | 2 | 1.0, 3.0 | NC |
| Glu↔AKG | 168 | 115, 341 | 202 | 106, 1895 | U |
| Pyrmit+Glu → Ala+AKG | 0.15 | 0.13, 0.16 | 0.15 | 0.14, 0.17 | NC |
| OAA+Glu → Asp+AKG | 0.18 | 0.16, 0.20 | 0.18 | 0.16, 0.20 | NC |