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. 2011 Apr 4;3(4):398–408. doi: 10.3390/toxins3040398

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© 2011 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Two modes of indirect detection of biotinylated detection mAbs. (a) Assay with capture mAb 1443; (b) Assay with capture mAb 1797. In each case, the background (BG) + 3 sd is indicated by the broken line, with the upper (dashed) line corresponding to the simultaneous addition of detection reagents, b-mAb + Ru(bpy)₃-streptavidin.