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. 2011 May 10;3(5):453–468. doi: 10.3390/toxins3050453

Figure 3.

Figure 3

RTA and ricin inhibit tunicamycin (Tm)-induced XBP1 splicing in HeLa cells. Cells were serum-starved for 2 h then treated ± RTA (1.0 µg/mL) and ± Tm (2.5 µg/mL) for 4 h. (A) Total RNA was analyzed by RT-PCR. U = unspliced XBP1; S = spliced XBP1; (B) qRT-PCR analysis of XBP1 expression corrected for β-actin and presented relative to Tm alone. Data were analyzed by one-way ANOVA with Bonferroni’s Multiple Comparison post-hoc test. **P < 0.001 and * P < 0.01 indicates different from Tm-treated cells. Bars represent mean ± S.E. of four experiments; (C) Cells were treated with 1.0 µg/mL of RTA (A), 1.0 µg/mL of RTB (B) or 0.1 µg/mL of ricin (R) ± 2.5 µg/mL Tm for 4 h; (D) Total protein synthesis in cells treated as in (C) for 4 h. Bars represent mean ± SE of 3 experiments.