Skip to main content
. 2011 May 10;3(5):453–468. doi: 10.3390/toxins3050453

Figure 4.

Figure 4

RTA does not affect thapsigargin (Tg)- and DTT-induced XBP1 splicing in HeLa cells. Cells were serum-starved for 2 h prior to treatment ± RTA (1.0 µg/mL) and ± Tg (1.0 µg/mL) for 4 h or ± RTA (1.0 µg/mL) for 4 h and ±DTT (2 mM) added over the last 30 min of treatment. (A) Total RNA was analyzed by RT-PCR as described in Materials and Methods. U = unspliced XBP1; S = spliced XBP1; (B, C) qRT-PCR analysis of XBP1 expression. Data were corrected for β-actin and presented relative to levels in cells treated with Tg or DTT alone. Data were analyzed by paired two-tailed t-test. * P < 0.05 indicates different from Tg-treated cells. Bars represent mean ± S.E. of four experiments.