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. 2011 May 10;3(5):453–468. doi: 10.3390/toxins3050453

Figure 6.

Figure 6

RTA inhibits DTT-induced phosphorylation of eIF2α in HeLa cells. Cells were serum-starved for 2 h prior to treatment with RTA (1 μg/mL) ± Tm (2.5 μg/mL) or Tg (1 μg/mL) for 4 h or with RTA (1 μg/mL) for 4 h with DTT (2 mM) added over the last 30 min of treatment. Total cell lysates (50 μg) were analyzed by SDS-PAGE. Membranes were immunoblotted with phospho-eIF2α antibody then for total eIF2α protein. (A) Representative blot is shown; (B) Data were quantified by densitometry. Phospho-eIF2-α was corrected for loading using total eIF2-α. Bars represent mean ± S.E. of 3 experiments. * P < 0.01 indicates different from DTT alone by paired two-tailed t-test.