Figure 7.

Inhibition of protein synthesis is enhanced in cells treated with both RTA and UPR-inducing agents in MAC-T cells but not in HeLa cells. MAC-T or HeLa cells were serum-starved for 2 h prior to treatment with RTA and Tm (2.5 μg/mL), Tg (1 μg/mL) or DTT (2 mM) for 4 h with the exception that for HeLa cells, DTT was added over the last 30 min of treatment. Total protein synthesis was determined by [³⁵S]methionine incorporation. Bars represent mean ± SE of 3 experiments.