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Primer design for quantitative RT-PCR.
| Gene | Forward Primer (5' to 3') | Reverse Primer (5' to 3') |
|---|---|---|
| Human spliced XBP-1 | TGCTGAGTCCGCAGCAGG | CGCCAGAATCCATGGGGAGA |
| Human total XBP-1 | GCAAGCGACAGCGCCT | TTTTCAGTTTCCTCCTCAGCG |
| Human β-actin | AATGTGGCCGAGGACTTTGATTGC | AGGATGGCAAGGGACTTCCTGTAA |
| Bovine spliced XBP-1 | TGCTGAGTCCGCAGCAGG | CATCAGAGTCCATGGGGAGA |
| Bovine total XBP-1 | GCAAGCGACAGCGCCT | TTTTCAGTTTCCTCCGCAGCG |
| Bovine cyclophilin | GAGCACTGGACAGAAAGGATTTGG | TGAAGTCACCAGCCTGGCACATAA |
