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Immunophenotypic analysis of mesenchymal stromal cells (MSCs) by flow cytometry. Cells were stained for CD73, CD90, CD105, and Stro-1, each of which identifies MSCs. CD34 is mildly positive, and the endothelial and hematopoietic cell markers CD31 and CD45 are negative. Control reactions were performed with irrelevant isotype IgG controls (gray lines). Typical results from individual subjects are shown. The percentage of positive cells is shown for each marker (mean±SD, n=3).
