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. 2011 Feb 22;20(11):1995–2007. doi: 10.1089/scd.2010.0494

FIG. 3.

FIG. 3.

Neonatal lung MSCs express higher levels of lung-specific transcription factors than cord blood MSCs. (A) mRNA expression of FoxF1, HoxA2, HoxA4, HoxA5, HoxB5, Gli2, and Wnt5A was assessed by qPCR. Data were normalized to GAPDH. (B–H). Immunocytochemistry of neonatal lung MSCs, fetal lung fibroblasts, and cord blood MSCs. Cells were probed for Wnt5a (red), FoxF1 (green), and Tbx3 (blue). Colocalization is white. (B) Neonatal lung MSCs. (C) MRC-5 fetal lung fibroblasts. (D) Cord blood MSCs. (E–G) Individual channels for Wnt5a, FoxF1, and Tbx3. (H) MRC-5 fetal lung fibroblasts stained with labeled control IgGs.