FIG. 5.

Differentiation capability of neonatal lung MSCs and fetal lung fibroblasts. Under adipogenic conditions, lipid droplets are revealed with staining by with oil red O (A–C). Osteogenic growth conditions create calcium deposits seen after staining with alizarin red (D–F). Chondrogenic differentiation promotes production of glycosaminoglycans, which are detected by staining with Alcian blue (G–I). Neonatal lung MSCs, but not fetal lung fibroblasts, differentiate along these lineages. We analyzed 3 MSC isolates, 3 fibroblast isolates, and 2 cord blood MSC isolates, and all yielded the same results.