FIG. 2.

Canonical WNT activity. (A) Induction of siamois was measured in dorsal marginal zone (DMZ) tissue cultured in the absence or presence of the indicated WNT1 concentration before RNA isolation and polymerase chain reaction (PCR) analysis during day 2 of culture. Highest levels of the WNT target gene siamois were obtained with 20 ng/mL WNT1. **P values between groups was <0.01. *P values between groups was <0.05. (B) Canonical WNT activity of WNT1 protein and the GSK3 inhibitor SB415286 was tested using QCE6 cells transfected with TOPflash-GFP and FOPflash-GFP reporter plasmids. The results are presented as fold-induction over nontreated control, with error bars corresponding to standard error of the mean.