FIG. 4.

Cardiac gene expression in DMZ tissue. DMZ tissue was cultured in the absence or presence of the indicated WNT1 concentration before RNA isolation and PCR analysis of cardiac (A) transcription factors and (B) muscle genes. (A) WNT1 treatment had little impact on of Islet-1 and GATA6 transcription but decreased expression of Tbx5, Tbx20, and Nkx2.5. (B) RNA levels for the cardiac-specific muscle proteins Actc, cMHCα, and cTnI were not significantly affected by WNT1 exposure. *P<0.05; **P<0.01 between WNT1-treated group and control.