FIG. 7.

Stem cell marker expression in response to canonical WNT signaling. (A) PCR analysis of DMZ tissue cultured without or in the presence of 20 ng/mL or 50 ng/mL WNT1. Treatment with 20 ng/mL WNT1 promoted an upregulation of both c-kit and oct25 expression. Expression of oct60 in DMZ explants was not affected by WNT1. (B) PCR analysis of cardiac tissue removed from nontreated and SB415286-treated embryos. SB415286 exposure promoted an upregulation of both c-kit and oct60 expression. In contrast, SB415286 did not stimulate expression of oct25. **P<0.01 between treated group and negative control.