Table 1.
Phenotype of Treated and Nontreated Embryos
| Reagent | Time of treatment | Total | Normal | A | B | C | D | Beating | % Contractile |
|---|---|---|---|---|---|---|---|---|---|
| Control | 160 | 150 | 7 | 2 | 0 | 1 | 159 | 99.4 | |
| SB415286 | 4 mM×10 min | 24 | 0 | 1 | 4 | 17 | 2 | 21 | 87.5 |
| SB415286 | 2 mM×30 min | 32 | 0 | 10 | 13 | 8 | 1 | 31 | 96.9 |
| SB415286 | 2 mM×1 h | 35 | 0 | 7 | 20 | 8 | 0 | 35 | 100 |
| SB415286 | 2 mM×2 h | 16 | 0 | 0 | 0 | 15 | 1 | 15 | 93.8 |
| SB415286 | 500 μM×24 h | 47 | 0 | 0 | 22 | 25 | 0 | 47 | 100 |
| LY-294,002 | 100 μM | 17 | 0 | 0 | 0 | 0 | 17 | 0 | 0 |
Embryos scored for the following phenotypes on day 4 of development: normal; type A, mild defect exhibited as a bend in the tail; type B, a highly anteriorized phenotype that was denoted by a severely truncated tail, but fully developed head; type C, severe malformation consisting of both a truncated tail and highly deformed head; and type D, viable but arrested development following treatment. Embryos were also scored for the presence of a beating heart.