Figure 1.

Cross-reactivities of antisera generated against GST-fusion proteins (fp) prepared from cDNA clones for AtSec21p and AtSec23p. Antibodies prepared against the 81-kD AtSec21p fusion protein recognized a 100-kD protein in the cytosolic fractions (c) and microsomal membranes (tm) of suspension-cultured Arabidopsis and cauliflower inflorescences, but not in yeast or pig brain. By contrast, antibodies against the 65-kD AtSec23p fusion protein recognized an 85-kD protein in the cytosol of all four organisms. Using equal amounts of protein (20 μg per lane), the signal for the cytosolic fractions was always stronger.