Figure 8.

Sephacryl S-1000 gel filtration of microsomal membranes extracted from cauliflower inflorescence. A microsomal membrane pellet was resuspended in extraction buffer and applied to the column, which was then eluted with the same buffer. Each fraction was monitored by western blotting with antibodies against marker proteins for the ER (BiP), Golgi apparatus (RGP), CCVs (β-adaptin), and plasma membrane (pATPase), as well as with AtSec21p and AtSec23p antibodies. The calibrating soluble protein was thyroglobulin (th).