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AtSec21p and AtSec23p antigens are bound to ER and Golgi membranes. Isopycnic Suc-density gradients of microsomal membrane fractions from cauliflower inflorescence were prepared under low (+EDTA) or high (+Mg2+) Mg2+ conditions. The fractions were probed by western blotting with the antibodies given in Figure 8 and tested for latent IDPase activity.
